What is a 1KB DNA ladder?

1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 – 10,000 base pairs (bp). The ladder consists of 13 double-stranded, blunt-end fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1).

How do you prepare 1 kb of DNA ladder?

If you want to prepare a working stock of the 1 kb ladder with loading dye, measure amount of ladder in the tube and add the loading dye accordingly. Use water to dilute your loading dye, in the ratio of 1 part ladder, 1 part buffer, and 4 parts ddH20 (if your loading buffer is 6X).

What is the highest DNA band size in 1 kb DNA ladder?

1 kb plus DNA ladders usually have about 14 purified DNA fragments with a reference band at 1000 and 3000 bp for easy orientation. Most 1 kb plus DNA ladders are provided with loading dye which allows a convenient detection of sharp bands after electrophoresis.

How much ladder do I add to gel?

For a standard electrophoresis system, we recommend loading 0.5 µg (20 µl) of the Fast DNA Ladder on the agarose gel. For a fast electrophoresis system (5 to 30 minutes separation), follow the system’s manufacturer recommendations: 5 to 20 µl load.

What does KB mean in gel electrophoresis?

kilobase
A measure of DNA chain length; 1000 nucleotide residues.

How many base pairs are in 1kb?

1000 base pairs
A kilobase (kb) is a unit of measurement in molecular biology equal to 1000 base pairs of DNA or RNA.

How do you dilute 100 bp ladder?

This ladder is preferred by our lab because of its broad range (100 bp to 10,000 bp) and even intervals. Its stock concentration is 1 µg/µl of DNA. The ladder is diluted to a 1:4 solution in water for use (3 parts water for 1 part ladder). To make 100 µl, 75 µl of water are combined with 25 µl of the DNA ladder.

What is the meaning of 100bp?

One basis point is equal to 1/100th of 1%, or 0.01%, or 0.0001, and is used to denote the percentage change in a financial instrument. The relationship between percentage changes and basis points can be summarized as follows: 1% change = 100 basis points and 0.01% = 1 basis point.

What does KB mean in genome?

Genome size refers to the amount of DNA contained in a haploid genome expressed either in terms of the number of base pairs, kilobases (1 kb = 1000 bp), or megabases (1 Mb = 1 000 000 bp), or as the mass of DNA in picograms (1 pg = 10−12 g). Genome sizes of bacteriophages and viruses range from about 2 kb to over 1 Mb.

What is the recommended load for 1 KB plus DNA ladder?

Quick-Load 1 kb Plus DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane. Suggested Load: 5 to 10 µl/lane. The following reagents are supplied with this product:

What is a 100 bp DNA ladder used for?

A 100 bp DNA ladder is a DNA size standard used for the sizing and quantification of double-stranded DNA of the size in the range of 100 bp to 1500 bp. These ladders consist of about 11 highly purified DNA fragments that form separate clear bands for the identification of other DNA fragments within that range.

What is Invitrogen 1 KB plus DNA ladder?

Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.

How many KB plus DNA ladder can be visualized by ethidium bromide?

1 kb Plus DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane. Suggested Load: 5 to 10 µl/lane. The following reagents are supplied with this product: